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Image Search Results
Journal: PNAS Nexus
Article Title: Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples
doi: 10.1093/pnasnexus/pgac028
Figure Lengend Snippet: Assay results: image processing, LOD, and analytical specificity. (A) After loading the sample and particles, each channel was imaged, and images were analyzed in ImageJ. The threshold was adjusted using the histogram tool until only the fluorescent particles were shown, followed by binarization. The individual areas of each white spot were collected, and any areas below 30 or above 10,000 pixels were excluded. The immunoagglutinated areas were summed in each image. (B) Standard curves were created using a series of SARS-CoV-2 spiked into saline gargle samples (10% saliva and 0.9% saline; n = 3 for each concentration, each time using a different chip). (C) Analytical specificity to SARS-CoV-2 was tested using negative and positive (1 pg/μL) SARS-CoV-2 samples, and influenza A/H1N1 (Ct value = 25–28), all in 10% saliva and 0.9% saline ( n = 3 for each sample, each time using a different chip).
Article Snippet: An additional test was performed to evaluate the analytical specificity using
Techniques: Saline, Concentration Assay